Your Challenge:
Circular Biomanufacturing

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Until now, our manufacturing processes have almost entirely been based on the use of newly, mined raw materials with not enough coming from the recycling of waste streams. This places an enormous burden on the environment and our society. In addition, dependencies remain in global supply chains that could be reduced through access to local materials.

Instead, we can create a circular economy in which new products are manufactured locally, using valorized waste streams as a source for raw materials, to build more sustainable and resilient production platforms.

To achieve this, biomanufacturing processes must be developed to market maturity and directly integrated with modern production processes. Scientific advances in recent years have produced new findings and methods that can significantly increase the performance of biomanufacturing processes and open up new application possibilities. Although alternative ways of producing a wide range of products to replace the conventional petrochemical or chemical manufacturing processes have gone to market, breakthroughs have so far only been achieved in niche applications. We need to reach the goal where the majority of bulk products are made through biomanufacturing processes that enable the use of locally available raw materials.

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The challenge: to develop an end-to-end prototype that processes various carbonaceous waste streams into new products as a continuous bioproduction process.

The prototype must demonstrate how carbonaceous waste streams can be processed and fed to microbes as food. The overall bioproduction process shall not use E. Coli or Saccharomyces cerevisiae and shall demonstrate continuous production over a period of at least 180 days during the Challenge. At the end of the process, at least three different products should be produced using a modern manufacturing process, such as additive manufacturing.

The Challenge runs over a three-year period. A panel of globally recognized experts will assist SPRIND in evaluating the applications and select up to eight teams to participate. During the Challenge period, teams further develop their bioproduction technology to achieve the Challenge goal.

Teams participating in this Challenge are fully challenged. SPRIND therefore provides intensive and individual support. This includes funding the teams with up to €1.5 million in Stage 1 of the Challenge, which started in November 2023. In order to unleash the full potential, SPRIND also provides a coach to accompany each team's work, advise them and network them. After one year and after two years, the jury reconvenes in each case to evaluate the interim status and decide which approaches have the greatest breakthrough innovation potential and which teams can prove themselves in the Challenge until the end.

Circular Biomanufacturing

In October 2024, the expert jury, on behalf of SPRIND, selected the participants for the second phase of the Circular Biomanufacturing Challenge. Over the next 12 months, the six teams will each receive up to 2 million euros to further develop their technology. The teams will also be supported by SPRIND, advised, and connected with additional experts and coaches. After one year, the jury will assess the progress of the developments and decide which teams will advance to the third stage of the Challenge.

Science Youtuber Jacob Beautemps introduces the Challenge teams at Breaking Lab

Breaking Lab "Circular Biomanufacturing"
Jury Circular Biomanufacturing
Patrick P. Rose, Petra Oyston, Clem Fortman, Deepti Tanjore, Julia Schüler, Rob Carlson, Ryan Ritterson. Not in picture: Michal Harari, Pae Wu

The Challenge is not restrictive in terms of the waste streams used. In addition to solid organic waste streams, the utilization of gas streams, for example, is not fundamentally excluded. However, the focus is on waste streams that have not been addressed or are difficult to access so far. The final prototype should be flexible and capable of operating using various waste streams/feedstocks.

The Challenge is only restrictive in the following aspects regarding the biotechnological processes used: 1. The process must enable continuous production of the synthesis product. 2. The use of E. Coli and Saccharomyces cerevisiae strains is excluded. A microbial or cell-free process can be employed.

The Challenge is not restrictive regarding the manufacturing processes used. However, individualization of the end products in the form of variant manufacturing must be possible.

The Challenge is not restrictive regarding the end products used. The selection of end products should illustrate the potential range and, if applicable, the platform nature of the technologies employed. The products from the biological process must be used in a form of advanced/additive manufacturing.

The project must have reached a Technology Readiness Level (TRL) of 3 at the start of the Challenge. You can refer to the Biomanufacturing Readiness Levels for guidance: https://academic.oup.com/jimb/article/49/5/kuac022/6712705. Please note that basic research will not be funded.

All Challenge teams are in close contact with SPRIND and the coaching team during the course of the challenge. This ensures a targeted innovation process in which emerging hurdles can be identified and addressed at an early stage. Teams must demonstrate that the minimum requirements of the call have been met by submitting stage reports. Furthermore, no detailed statements on the use of funding are required.

The target values for Stage 1 represent the minimum that must be achieved to be considered for Stage 2.

All expenses that serve to achieve the Challenge goal can be financed with SPRIND funds. This can include, for example, personnel costs, equipment and materials or rent.

Applications by project consortia are permissible. The leading consortium partner must be identifiable as the sole contracting partner with SPRIND and have its headquarters in the European Union, European Free Trade Association (EFTA), the United Kingdom, or Israel.

The intellectual property rights created by the teams during the Challenge remain with the teams. SPRIND receives a free and non-exclusive right to use the results found. The teams undertake to grant licences to third parties at standard market conditions. Details can be found in the Participation agreement which will be published alongside the Call for Submissions.

SPRIND is committed to supporting innovators in implementing breakthrough innovations. If SPRIND identifies breakthrough innovation potential in the teams during the Challenge, their work can continue to be supported after the Challenge has ended.

An expert team from SPRIND will make an initial selection from the received applications. The final decision on admission to the Challenge will be made on the basis of the application and the pitch in front of a jury of scientists, industry experts and investors. The pitch days are scheduled to take place at the end of October 2023 in Leipzig.

The application deadline is September 17, 2023, at midnight CET (Central European Time).

Do you have further questions?

Please feel free to contact us at challenge@sprind.org.

Jano Costard, Challenge Officer
Jano Costard, Challenge Officer

NEW CHEMICALS FROM WASTE

How EveryCarbon is upgrading biogas plants

Dr Sebastian Beblawy originally wanted to be a chemistry and biology teacher. But he didn't like the biology lectures during his studies. I really found it awful, they were so boring, the 35-year-old recalls. That all changed when he joined Prof. Johannes Gescher's research group: It was about a completely different kind of biology: sustainability, technology and wastewater. That fascinated me. Instead of doing a teacher traineeship, Sebastian Beblawy went on to do a PhD, devoting himself entirely to sustainable biotechnology. But neither his academic career nor a subsequent career as a consultant in the biotechnology industry fully satisfied him: Eventually, I reached a point where I returned to my PhD supervisor and said I was ready to start my own company. Coincidentally, the SPRIND Circular Biomanufacturing Challenge was announced at the same time. I knew it was my call, says Sebastian Beblawy, and then I was accepted. I resigned the very next day.

Within a very short period of time, Sebastian Beblawy founded the company EveryCarbon, which now employs six people. Only six weeks passed between the approval of the funding and the start of the project, says a satisfied Sebastian Beblawy who continues to work closely with the Hamburg University of Technology.
EveryCarbon
2,3-butanediol can be used, for example, as a substitute for glycerine or as a solvent for paints. It is also used as a raw material for plastics. There are a total of three naturally occurring variants of 2,3-butanediol. Chemically, they are the same product, but the atoms are arranged differently in the molecule depending on the variant.

Traditional chemical catalysis usually produces a mixture or a specific variant, explains Sebastian Beblawy. But what makes EveryCarbon different is that, thanks to the bacteria, the company can control exactly which of the three variants to produce, or which composition it wants. Large chemical companies cannot compete with this flexibility for cost reasons.
EveryCarbon
The first pilot plant is due to be connected to a sewage treatment plant near Stuttgart in 2025. We want to show that the process is scalable and can produce relevant quantities of product, says the CEO. He explains the key advantage of EveryCarbon: With our technology, we can retrofit existing infrastructure and make it more lucrative. We talk about the 'carbon upgrade factor', which means we can increase the value per carbon by up to 80 times compared to biogas.

We want to show that the process is scalable and can produce relevant quantities of product.

EveryCarbon
The young company aims to combine traditional waste disposal and productive biotechnology in a single process. In concrete terms, this means that in the first stage of the process, the company homogenises various waste streams, including household waste and industrial waste from food production, using wastewater as the process medium. The first stage of the process is not the best in terms of odour, admits Sebastian Beblawy. After anaerobic digestion, the waste mixture is separated into a liquid and a solid phase. In the solid phase, the first process step is repeated; particularly stubborn waste is additionally treated.
The second stage of the process focuses entirely on the liquid phase of the waste. We use a genetically modified bacterial strain that can cope well with the complex and fluctuating process stream and produce a pure chemical substance from it: 2,3-butanediol, explains the microbiologist.
EveryCarbon
We can produce 2,3-butanediol, which behaves very differently mechanically and thermally than the classic mixture from chemical catalysis. For example, we can produce molecules with a much higher melting point, says Sebastian Beblawy, proudly explaining the advantages. The company has two strategies: The first is to replace existing materials on a one-to-one basis, and the second is to create new materials with new functions that can still be returned to the normal plastics cycle. In reality, however, recycling should only take place after many years. For Sebastian Beblawy, it is important that the end products are durable so that the original carbon in the waste is retained for as long as possible: One future application would be building foams for the construction industry, for example.
Every Carbon
EveryCarbon
The founder grows alongside the challenges of running a business: My way of thinking has changed a lot. Above all, you have to get used to entrepreneurial thinking. SPRIND supports him in this. The Challenge is very much focused on the commercialisation of technologies, Sebastian Beblawy explains, adding: It starts with the fact that the funding is not given as a grant, but as a pre-commercial contract. Whether you receive follow-up funding depends on whether you meet the terms of the contract. So you are guided towards commercial orientation from day one.
For Sebastian Beblawy, the challenge also led to personal growth: I learned about myself that I am creative chaos. I have a lot of ideas, but I need people around me who are more organised and structured. He found that balance in his team. We’ve developed a team dynamic that works really well and and makes the whole process fun.
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